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Quantification of positively stained cells and mean fluorescence intensity was performed using ImageJ software (National Institutes of Health, Immuno-electron microscopy Skin tissues were fixed in 4% paraformaldehyde (163-20145, FUJIFILM Wako Pure Chemical Corporation) for 30 minutes at room temperature, and then washed three times in PBS (5 min each)
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If its a digital or subungual melanoma, it may result in amputation of the distal extremity. Fortunately, Dr
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