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Although we have confirmed the accumulation of Lf-LPs by fluorescently labelled LPs, we did not directly evaluate the concentration of ASX in brain

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A cofactor in the methionine production is tetrahydrofolate (THF) that transfers a methyl group to the -SH group of homocysteine, the precursor of methionine

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Finally, the membranes were immersed in enhanced chemiluminescence reagents (Thermo Fisher Scientific) and the blots were visualized using a luminescence analyzer (Fusion Solo, Vilber Lourmat, France)