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The approach is also compatible with the controlled production in stirred tank bioreactors as recently demonstrated for cardiac and hematopoietic lineages 77,78 and will provide the field with a potentially game-changing resource to allow the development of clinical as well as screening platforms where the requirement will be in the millions of organoids rather than todays laboratory scale

The optimum DDA concentration was 2.5% based on the sizes, PDI, and zeta potentials of the formulations following Dox loading

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