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Taken together, we successfully engineered Lp to display an active tetramer streptavidin protein on its bacterial surface and enabled the surface loading of biotinylated molecules in the engineered strain

We argue that lactyl-CoA is in the l form, because it can be labeled by isotopic l-lactate and glucose, and because l-lactate is the major enantiomer in cells (present in millimolar concentration 31 compared to about 10 M for d-lactate 32 )

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[Google Scholar] Chen, Y

The cells were stained in FACS buffer (PBS containing 0.5% bovine serum albumin and 0.05% sodium azide) with fluorescently labelled antibodies from BioLegend (CD11c-APC/Cy7, CD11b-BV421, CD40-FITC, CD80-APC, CD86-PE, CD8-PE/Cy7, CD4-PerCp/Cy5.5, CD69-PE, CD25-APC, CD3-BV421, CD44-BD427, CD45.1-FITC, CD3-PerCp/Cy5.5, and CD44-FITC) or Tonbo Biosciences (HLA-DR-PerCp/Cy5.5) to identify DC and T cells

[DOI] [PMC free article] [PubMed] [Google Scholar] 59.Lillo-Moya J, Rojas-Sol C, Muoz-Salamanca D, Panieri E, Saso L, Rodrigo R