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Insert the needle at a 45-90 degree angle (90 degrees for areas with more subcutaneous fat, 45 degrees for leaner areas)

The CDC/NIH Biosafety in Microbiological and Biomedical Laboratories manual emphasizes protocol-driven risk assessment and notes that no single document can identify every possible risk combination

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Although daily flushing or draining might seem excessive, scheduled purges can keep water from lingering in dead zones

Mistake 4: poor reconstitution and storage Using tap water instead of bacteriostatic water

doi: 10.1016/0002-9416(82)90033-1