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It is imperative to thoroughly investigate and understand these factors to regulate them effectively, as they may hold the secret to preventing or treating PD [116,117,118]

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Off-target effects and specificity Another significant challenge in implementing RNAi in crops is the possibility of off-target effects, where small interfering RNAs (siRNAs) or microRNAs (miRNAs) derived from double-stranded RNA (dsRNA) attach to and silence non-target genes

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