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In the absence of PDIA6 droplets, all-Cys/Ser proinsulin, which cannot form disulfide bonds, was found to be highly prone to aggregation, as monitored by ThT fluorescence

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This results in the transcriptional upregulation of pro-inflammatory cytokines (IL-1, TNF-, IL-6) [32], chemokines (CXCL8/IL-8, CCL2), antimicrobial peptides, and co-stimulatory molecules that enhance immune cell recruitment and activation [34]

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