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A FACS Canto II flow cytometer (BD Biosciences) was used for the analysis employing Kaluza software (Beckman Coulter, Brea, CA, USA)

, poly ADP ribose polymerase [PARP]), together leading to an increase in the NADH/NAD + ratio and promoting ROS production by mitochondrial respiratory complex I due to NADH overload, eventually resulting in reductive stress (38, 105, 107)

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doi: 10.1002/mnfr.200800177

Heavy Metals: Mercury, Lead, and Oxidative Damage Toxic metals, such as mercury and lead, can cause significant damage to mitochondrial health and cellular function

Vitamin D has been found to have therapeutic effects in models of epithelial barrier dysfunction, characteristic of COPD, caused by cigarette smoke and toluene diisocyanate [22, 23]