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doi: 10.1200/JCO.2021.39.15_suppl.9074
PapierKBradburyKEBalkwillABarnesISmith-ByrneKGunterMJet al
Briefly, adult fat bodies were dissected in 1 phosphate buffered saline (PBS), fixed 20 min in 4% paraformaldehyde at room temperature (RT), rinsed 5 in 1 PBS, then permeabilized for 30 min at RT in 1xPBS + 0.3% Triton X-100
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Mechanical shear can disrupt the disulphide bond and destabilise the loop conformation
