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Plateaus are a normal and expected part of the process. Vincent Bottaro, PA-C Weight loss is not a linear journey

Light protection keeps product in original vial with minimized light exposure, while moisture protection requires sealed, dry conditions

8 h post-transfection, medium was changed into 2 ml of DMEM (Gibco, 41966-052) supplemented with 10% (v/v) FCS

Jiamworanunkul S (2019) Effective antioxidant production through submerged fermentation of edible mushrooms

Molecular modeling of GGP1 The predicted protein structures of GGP1 and GGP3 (version 2) were obtained from the AlphaFold Protein Structure Database ( and The initial molecular model of GSB was generated using PCMODEL (Serena Software, Bloomington, IN, USA)

Once internal standard concentrations were obtained, the peak areas of the unlabelled metabolites in the samples of culture medium were compared with the peak area of the quantified internal standard to determine the metabolite concentration in the sample