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An extremely useful feature offered by lipid isomer analysis, is the relative quantitation achieved at high precisions without requiring the use of lipid standards, which is not readily available 25,26

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Although the concentration of CDCA and CA (both high potency and low potency FXR agonists) increased in HFD-fed mice on day 10 after infection, the proportion of this increase was not as large and it ranged from 213-fold, compared to RD-fed mice (Fig