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2014 Feb Tonje Holte Stea, Mohammad Azam Mansoor, Margareta Wandel, Solveig Uglem, Wenche Frlich

The role of thiamine deficiency in alcoholic brain disease

doi: 10.1016/j.yexcr.2015.04.004

Further, comparing the overall structure of the mature and T391A precursor of EcGGT demonstrated that backbone atoms in the core regions of both the proteins remain unchanged, while significant conformational changes occurred proximal to the active site (Okada et al., 2007)

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The brains were then homogenized using a Dounce glass/glass homogenizer by 20 strokes with the loose pestle followed by 20 strokes with the tight pestle