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Participants were excluded from the study if they suffered from the following diseases: alcohol use disorder, diabetes mellitus, kidney or bladder stones, kidney disease, liver disease, or stomach ulcers

The protein concentration in the supernatant was determined by bicinchoninic acid assay (BCA) (Pierce)

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Vitamin B9 (Folate) - Important for DNA synthesis and repair, crucial during periods of rapid growth

Vitamin B12 is essential to the formation of Folic acid

Blocking caspase activity prevents transsynaptic neuronal apoptosis and the loss of inhibition in lamina II of the dorsal horn after peripheral nerve injury