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Insoluble material was pelleted by centrifugation at 10,000 rpm for 10 min

10.1016/j.blre.2019.05.006 139 NihoN.ShibutaniM.TamuraT.ToyodaK.UneyamaC.TakahashiN.et al (2001)

Tirzepatide is studied as a dual agonistmeaning it activates both the GIP receptor (one incretin pathway) and the GLP-1 receptor (another incretin pathway)

The disruption of mechanisms such as oligodendrocyte maturation and migration, myelin phagocytosis, myelin sheath renewal and the inflammation itself are consonant with the full pathological progression of demyelination

doi:10.1093/eurheartj/ehr482 Nishioka K, Hidaka T, Nakamura S, et al

Absence of subjective effects does not necessarily mean the peptide is not producing cellular benefits
