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Cells were resuspended in 5 to 10 mL of lysis buffer (20 mM sodium phosphate pH 7.5, 200 mM NaCl, 0.05% (w/v) Tween-20, 2 mM MgCl 2 , 0.5 mM TCEP, +400 U DNAse I, 12.5 mM imidazole and a tablet of Complete EDTA-free protease inhibitor cocktail, Roche, per 100 mL of buffer) per gram of cells

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This work was supported by grants [AGL2012-31968 and AGL2016-78662-R] from Ministerio de Economa y Competitividad, Spanish Government and European Research Council Starting Grant under Grant [BacBio 637971]

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( h ) Confocal images of uninfected and infected FRCs

The present study aims to investigate the causal effect of meteorological and climatic variables such as rainfall, humidity, elevation, normalized difference vegetation index (NDVI), population etc