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Cells were washed three times in 500 l PBS, lysed with 0.2% Triton X-100 at 37 C for 10 min and plated on LB agar

To view a copy of this licence, visit About this article Cite this article Ryan, D.H., Lingvay, I., Deanfield, J

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Chemical reactions in the body, therefore adequate cobalt and selenium are required for maintenance of condition, growth and milk and wool production

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