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Death was determined based on standardized criteria including the absence of respiration, absence of heartbeat, loss of pupillary light reflex, and absence of reflexes, with scavenging and operator protection measures in place

RNA was extracted using the Qiagen RNeasy Kit (Cat# 74004)

Trounson A

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It does this by activating endothelial nitric oxide synthase, which is responsible for the production of nitric oxide a potent vasodilator

Psychol Rev (2019) 126:791816