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Flow cytometry analysis To stain human ILC2s and mouse ILC2s, single cell suspensions prepared from nasal polyp tissues, induced sputum and mouse lung tissues were treated with a human BD Fc Block (BD Bioscience, USA) or mouse BD Fc Block anti-mouse CD16/CD32 mAb 2.4G2 (BD bioscience), and then stained with specific cell surface markers in staining buffer (PBS supplemented with 1% FBS and 5 mM EDTA) for 30 min on ice

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Their antimicrobial effect is largely attributed to their hydrophobicity, which allows them to disrupt the permeability of bacterial cell membranes [89, 90]

Quantifying intracellular rates of glycolytic and oxidative ATP production and consumption using extracellular flux measurements

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13 C 3 -serine, 141.667 mM, 3 l min 1 , 2 h