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Obtained RNA samples were eluted in 30 L of RNAse-free water

Physical and Emotional Withdrawal: A critical aspect involves recognizing withdrawal symptoms, both physical and emotional, when the individual is not consuming alcohol

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Detoxification Glutathione binds to heavy metals, pollutants, and other harmful substances to render them water-soluble for easier excretion

After the treatment period, the culture medium of each well was replaced by 0.2 ml of calcein AM solution (Sigma-Aldrich) at 2 M in phosphate buffer [PBS 1X] and the culture plates were put into the incubator for 30 minutes before fluorescence reading using a Synergy H1 microplate reader (Biotek, Winooski, VT, USA