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ATP levels were determined by measuring fluorescence at Ex/Em = 535/587 nm, using a multi-well plate reader (Synergy H1, BioTek Instruments, Inc., Warsaw, Poland)

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Namely, the retina and the brain share many supporting functional and structural similarities [138], and as one of the longest central nerves, the optic nerve connects the retina to the thalamic brain nuclei [31]

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