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An aliquot from the wells containing the most active LacAnc100 and LaccID clones was inoculated in 3 ml of YPD (10 g l 1 yeast extract (Gibco, 212750), 20 g l 1 peptone (Gibco, 211677), 20 g l 1 d-glucose (Thermo Scientific, 410955000) and 25 g ml 1 chloramphenicol (Merck, C0378)) and incubated at 30 C and 225 rpm for 24 h

10.1016/j.jtherbio.2021.102943 J Therm Biol

C-terminal amidation replaces the terminal carboxyl group with an amide, protecting against carboxypeptidase degradation

Unable to absorb vitamins Methylated vitamins are the active versions of vitamins which your body can readily make use of, while un-methylated vitamins must go through a conversion process before your body can actually use them

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The wider picture is, once again, one of a connected set rather than a collection of soloists