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Expression and purification of Ulp1-protease (SUMO-protease) The ubiquitin-like-specific protease 1 (ULP1) (AA 403-621) from Saccharomyces cerevisiae containing an N-terminal His 6 -tag (LifeSensors Inc., Malvern, USA) (pET28b, Kan R ) was transformed into the E

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