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For BrdU staining, DNA strands were denatured for 1 h at room temperature using 2 M HCL, prior to primary antibody incubation

10.1038/s41570-021-00310-4 10.1038/s41570-021-00310-4 [DOI] [PubMed] [Google Scholar] Michel F, Bonnefont-Rousselot D, Mas E, Drai J, Thrond P (2008) Biomarqueurs de la peroxydation lipidique: aspects analytiques [Biomarkers of lipid peroxidation: analytical aspects]

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Sorli, C., Harashima, S

Meanwhile, we detected the HIF-1, a key protein in the mechanism of APAP-induced liver injury 35 and found that 5-HT could decrease the expression of HIF-1 by which 5-HT contributed to relieve of APAP-induced hepatotoxicity (Figure 8D and 8E)