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For example: 2.5 mg from a 5 mg/mL vial = 50 units 2.5 mg from a 10 mg/mL vial = 25 units 2.5 mg from a 20 mg/mL vial = 12.5 units This is why patients should never copy someone elses dosing instructions

doi:10.1038/nrm778

Add 1,000 L (1 mL) of reconstitution solvent to the 1 mg vial This yields a 1 mg/mL (1,000 g/mL) stock solution To achieve 100 g/mL working stock: Dilute 100 L of 1 mg/mL stock into 900 L of PBS or cell culture medium yields 100 g/mL in 1 mL To achieve 10 ng/mL in a 5 mL experiment: Take 0.5 L of 100 g/mL working stock + 4,999.5 L of medium Alternatively: dilute to an intermediate concentration to reduce pipetting error Use a dilution series approach (stock working stock final experimental concentration) to minimize pipetting inaccuracy at nanogram-level concentrations

Another study revealed a more specific mechanism underlying RV failure: under PAH conditions, circulating CCR2 + macrophages selectively infiltrate the RV and express high levels of the NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome, leading to mitochondrial dysfunction in cardiomyocytes

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