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The underlying discrepancy may be attributed to differences in cellular type and metabolic state: tumor cells such as HT-1080 often rely predominantly on glycolysis (the Warburg effect), rendering mitochondrial function secondary

After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min

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368:8589 Doll S, Freitas FP, Shah R, Aldrovandi M, da Silva MC, Ingold I, Goya Grocin A, Xavier da Silva TN, Panzilius E et al (2019) FSP1 is a glutathione-independent ferroptosis suppressor

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Eur J Med Chem 200:112442