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However, careful consideration of their limitations and optimization of experimental conditions are necessary, particularly when working with complex biofluid samples, to ensure accurate and reliable results

After 24 h, treated biofilm supernatant was washed once with 1 PBS followed by addition of 200 L of 1 PBS plus 15 L resazurin 0.05% w/v solution, and incubated for a further 24 h, at 37C, 150 rpm

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In the study of triple-negative breast cancer (TNBC), researchers found that hybridized BLG@TPGS NPs were prepared by doping the multipath energy inhibitors berberine (BBR) and lonidamine (LND) as well as the chemotherapeutic agent garcinia cambogia (GA), which inherited the mitochondria-targeting ability of BBR and accurately accumulated in the mitochondria, then induced starvation therapy, which effectively eradicated cancer cells by coordinating the shutdown of tumor cells through the three-tendency strategy to cut off the mitochondrial respiration, glycolysis and glutamine metabolism [223]

After injection of human BMSCs into the NP of a bovine IDD model, BMSC apoptosis and migration were regularly detected, and the results showed that the IDD environment did not affect the activity of BMSCs and allowed the cells to migrate to the injured site