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doi: 10.1002/ibd.21814

Capillary needles for RNA injection were made using a PC-10 micropipette puller (Narishige), and each oocyte was microinjected with 25 ng of RNA using the Nanoject III (Drummond Scientific), followed by incubation at 16C for 3 d in Modified Barths Solution (EcoCyte Bioscience) with 10 IU ml/ml gentamicin (Invitrogen)