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After blocking, they were processed with specific primary antibodies at 4 C overnight and with secondary antibodies in next day

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They found that using the potent copper ionophore elesclomol as the test substance, elesclomol-induced human renal smooth muscle tumor G402 cell death did not involve the activation of caspase-3

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We found that treating HG-3 and MEC-1 cells with CB-839 increased LC3-I to LC3-II protein conversion and p62 protein degradation ( Figure 4A )

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