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Take a new syringe from the package (syringes are single use only, call or visit us to get refills on syringes when needed) Draw up 0.25ml of the red Vitamin B12 solution (measure from the top of the black plunger). Pull up the skin between the shoulder blades to create a skin tent Insert the whole needle into the skin (pointing downward towards the body), make sure the needle has not exited the skin on the other size of the skin tent

Both sites contain sufficient muscle mass to safely accommodate injection depth without striking bone or critical nerves

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Drinking sufficient water the day before your test, avoiding excessive caffeine (which has diuretic effects), and ensuring you are not in a dehydrated state from missed electrolytes are all simple preparatory steps

10.1016/j.cell.2011.06.001 Cell 122 McManusR