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In laboratory settings, 5-Amino-1MQ has been studied for its ability to reduce NNMT-mediated methylation of nicotinamide, which influences the availability of methyl groups and the cellular NAD+ pool

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5-Amino-1MQ has been investigated in preclinical models as a selective inhibitor of NNMT, an enzyme that catalyses the methylation of nicotinamide and has been referenced in the literature in connection with cellular energy metabolism, NAD + -associated signalling pathways, and energy homeostasis