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The amount and purity were tested using a Nanodrop (UV-Vis spectrophotometer Q5000/USA), and the integrity was evaluated using gel electrophoresis

Biochem Biophys Res Commun 325:109116 Sato H, Shiiya A, Kimata M, Maebara K, Tamba M, Sakakura Y, Makino N, Sugiyama F, Yagami K, Moriguchi T et al (2005) Redox imbalance in cystine/glutamate transporter-deficient mice

(79) demonstrated that the exosomal miR-199a-5p induces lipid build-up in the liver through a down-regulation of its target gene, hepatic Mammalian sterile 20-like kinase 1 ( MST1 )

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The fix: Start with 0.5-1% concentration