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A large protein complex of the inner membrane with a dual role: maintenance of the cristae architecture of the inner membrane and the formation of contact sites between the inner and the outer membranes

Zhou, Jiajia

Proteins in gel pieces were reduced and alkylated using a final concentration of 5 mM dithithreitol and 15 mM iodoacetamide before digestion with Trypsin (Promega) at 25 ng l 1 in 50 mM ammonium bicarbonate, first for 1 h on ice and then for 16 h at 37 C using shaking incubator

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Our study has some limitations