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Thiol-containing compounds (RSH) react with DTNB to form mixed disulfides (RS-S-DTNB), where the thiol group replaces one of the nitrobenzoic acid moieties in DTNB

Over time, telomeres shorten, ultimately limiting cellular replicative capacitya central feature of biological aging

The incubation was continued in singlet and at varying time points after addition of phase I cofactors (0, 10, 30, 60, 120 min), the reactions were quenched with 0.5 mL (1:1) of methanol containing 0.2% formic acid, 4 mM ammonium acetate (Isoxazole 2 only), and 100 ng/mL N-benzyl-benzamide internal standard (0.1%FA, 2 mM NH 4 acetate, and 50 ng/mL final concentration)

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these results suggest that BPC-157 has no in vitro mutagenic or genotoxic effects