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To generate a standard curve of known amino acid content, a dilution series from 0 to 34 nmol of alanine (FW 89.1 g mol 1

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Devos D, Devos D, Cabantchik ZI et al (2020) Conservative iron chelation for neurodegenerative diseases such as Parkinsons disease and amyotrophic lateral sclerosis

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doi:10.1002/cmdc.200700015

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For the same reason above, it is possible that dehydro-alanine (acrylate) glutathione can inactivate the binding capacity of GCLC to form the holo-enzyme

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