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Implementing a clinical immunogenicity strategy using preclinical risk assessment outputs

Mechanistically, the incubation of hemin with thiols (GSH, NAC, cysteine, mercaptoethanol, and thioglycolic acid) provokes major spectrophotometric changes in hemin despite the presence of low-affinity binders in serum [222]

Our aim was to investigate the effect of UA on the metabolism of endogenous antioxidant glutathione by estimating UA induced changes in the expression of glutamate-cysteine ligase, glutathione peroxidase, glutathione reductase, and glutathione synthetase in J744A.1 mouse macrophage cell line

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and oxidation via the cytochrome, P450-dependent, mixed-function oxidase enzyme pathway to form a reactive intermediate metabolite, which conjugates with glutathione and is then further metabolized to form cysteine and mercapturic acid conjugates