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For data-dependent scanning mode, MS/MS normalized collision energy was set to 20, activation Q 0.25, activation time 25 ms, with a 1 m /z isolation window, while a reject mass list was generated by injecting blank samples consisting in a mixture of acetonitrile:water:formic acid (50:49.9:0.1, v/v/v)

Subsequently, 200 l of fresh YPD medium was added to promote biofilm growth for another 21 h at 37 C

Antonoff MB, Correa A, Sepesi B, Nguyen Q, Walsh G, Swisher S, Vaporciyan A, Mehran R, Hofstetter W, Rice D

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OS = Osteosarcoma