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3 The relationship between circadian rhythms and intervertebral disc physiology 3.1 The IVD is a tissue with diurnal rhythmic behavior Diurnal rhythms refer to 24-h biological oscillations that are synchronized with external cues, such as light-dark cycles, while CRs denote endogenous oscillations that persist under constant environmental conditions

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The culture medium was removed, and cells were washed with 200 L of phosphate-free DMEM, and treated with DMEM containing 1 mM phosphate and DMSO/SC-919 (1 M) for 4 h
