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The samples were centrifuged at RT for 1 min at 12,000 g after the FastPrep step

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Adjustments were made for individual traditional cardiac risk factors (age, gender, diabetes mellitus, systolic blood pressure, former or current cigarette smoking, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol), extent of CAD, left ventricular ejection fraction, history of MI, baseline medications (aspirin, statins, -blockers, and ACE inhibitors), and renal function by estimated creatinine clearance

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After February, the case load decreased each month, and in June, at the close of the RFC program, the case load was 102,344 or 10 per cent of the state's population