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The Hordaland Homocysteine Study

Flow cytometry was performed using a BD Fortessa instrument (BD) or NovoCyte Quanteon and data were analyzed using FlowJo v10.6.1 software (Tree Star)

Membranes were washed thrice in PBS/Tween/BSA and bound proteins detected with mouse anti-Penta-His antibodies (1:10,000

GSH and GSSG levels were determined as the difference in low molecular weight thiols

Poly-ubiquitin chains are particularly important, as they often label target proteins to be sent to the proteasome for degradation, which is a major mechanism by which cells remove unwanted or damaged proteins
