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A negative control (ddH O replacing template DNA) was included in each PCR run to monitor potential contamination
Kokoeva MV, Yin H, Flier JS
doi: 10.1016/j.freeradbiomed.2011.05.017
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Whenever a test substance is considered mutagenic according to the above-mentioned criteria, the ratio of small versus large colonies is used to differentiate point mutations from clastogenic effects
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