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Cellular ROS detection The cellular ROS was detected using a Cellular Reactive Oxygen Species Detection Assay Kit (Abcam ab186029) according to the manufacturers protocol

Mitchell, KG, Parra ER, Nelson DB, Corsini E, Zhang J, Vaporciyan AA, Hofstetter WL, Mehran RJ, Swisher SG, Rice DC , Sepesi B, Walsh GL, Behrens MC, Kalhor N, Weissferdt A, Moran C, Skoulidis F, Wistuba II, Fujimoto J, Roth, JA, Antonoff MB

& Wu, S

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27 Besides, nanostructures are always employed to sustain the release of encapsulated compounds in order to reduce dose and dosing frequency, thereby increasing patient compliance and minimizing side effects