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Treatment options and practical self-care When carnitine deficiency is confirmed, treatment usually focuses on the cause as well as replacement

Oil red staining of tissue frozen sections First, the tissue sample was cut to an appropriate size (approximately 2cmx2cmx1cm), embedded with biological glue, wrapped with aluminum foil, and marked with isoprene

2002;361(Pt 3):41729

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2.7 Aortic ring assay The mouse aortic ring assay was conducted according to a previously described method ( 2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes