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Stancill JS, Corbett JA

Diffraction quality crystals of Rg GUS (3 mg/ml) and Bf GUS (15 mg/ml) were grown in 100 mM sodium citrate buffer, pH 4.3, 100 mM sodium citrate salt, 22% (w/v) PEG 3350 at 4 C or in 250 mM DL-malic acid, pH 7.0, 20% (w/v) PEG 3350 at room temperature, respectively
For the mTOR rescue experiment, SYK-deficient SC (shSYK-1) was treated with 5 M MHY1485 (MCE, Cat#HY-B0795) for 24 h
The HRMS analysis was performed in full scan mode, collecting data in the range of m/z 212.5750 using ionization parameters previously described in detail 31

Cancer Invest 11(2):229-34, 1993
[DOI] [PMC free article] [PubMed] [Google Scholar] 234.Ishikawa Y., Hashizume K., Kishimoto S., Tezuka Y., Nishigori H., Yamamoto N., et al