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Studies have found that supplementing 5002,000 mg of vitamin C per day can support glutathione recycling and help replenish the antioxidant pool 27

Briefly, specimens were incubated at 4 C for 48 h with primary antibodies at a 1:200 dilution: rabbit polyclonal anti-Myosin VIIa (#25-6790, Proteus Biosciences, US), Phalloidin (#CA1640 and #CA1670, Solarbio, China), mouse monoclonal anti-SIRT1 (#Ab110304, Abcam, UK), mouse monoclonal anti-3-NT (#N5538, Sigma-Aldrich, US), rabbit polyclonal anti-4-HNE (#46,545, Abcam, UK), mouse monoclonal anti-NF (#GB12144, Servicebo, China), and rabbit anti-Phospho-AMPK1 antibody (#AP0871, Abclonal, China)

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The high content of Cu + () ions in neurons (from 0.1 M to 1.3 M) encourages Cu 2+ -catalyzed protein oxidation and may be associated with a toxic increase in peroxidase activity, which can generate CO 3 through HOOCO 2 and thiol oxidase activity [14]

Mercury causes severe brain abnormalities, for example in Minamata disease