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Quantitative real-time PCR Cells and tissues were first lysed using Trizol, then the total RNA was extracted with RNA Easy Fast Tissue/Cell Kit (Tiangen, city, ST, Ctry

As one parent had already been recruited to the Project GEHA (genetics of healthy aging), they were referred to as GEHA offspring (GO)

The rupture of cell membranes within the tissue results in the combination of polyphenol substrates with polyphenol peroxidases, initiating browning reactions

The human ACE gene expresses two distinct isoforms, the somatic ACE (sACE) and testicular ACE (tACE) 294

The placenta experiences a heightened level of OS in certain pathologic conditions of pregnancies, including gestational diabetes, fetal growth restriction, preeclampsia and miscarriage [64,65,66]