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The animals were first adapted to testing environment after a shortened pellet (10.3 cm), their usual diet, was administered in the cylinder

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Virz GM, Milan Manani S, Marturano D, Clementi A, Lerco S, Tantillo I, et al

The His6-SUMO tag was cleaved by ULP1 treatment, and the tag-free protein was fractioned with Ni-NTA column and Superdex 75size exclusion column (GE Healthcare) with 25 mM HEPES (pH 8.0), 300 mM NaCl, and 1% glycerol
Folate is an important cofactor in one-carbon metabolism of amino acids, nucleotides, and other metabolites [22]
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