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Generation of the IscE1 meganuclease-based Tg( tg:nrf2a_T2A_mKO2nls ) transgenic zebrafish line The Gateway cloning technologybased Tol2kit (Kwan et al, 2007) was used to generate a rescue construct in which the functional nrf2a coding sequence fused to a nuclear-localized mKO2 fluorescent protein with a viral T2A linker peptide (Kim et al, 2011) is expressed under the control of a fragment of the zebrafish tg gene promoter previously shown to allow thyroid-specific transgene expression (Opitz et al, 2012)

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Results In total, 100 COPD patients were enrolled from 10 hospitals

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Immune System Support Boosts immune function: Glutathione is critical for proper immune cell function and can help strengthen the body's defenses against infections