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3 Oxidative stress Oxidative stress is a disbalance caused by excessive production of prooxidant substances such as ROS and RNS and/or the antioxidant systems working inefficiently ( in vivo primarily by the mitochondrial respiratory chain during aerobic metabolism ( via a reaction between nitric oxide (NO) and superoxide, and nitrosoperoxycarbonate, generated via a reaction between peroxynitrite and carbon dioxide

HAP1 cellsfrom KJ Patels laboratory, originally from Haplogenwere grown in Iscoves Modified Dulbeccos Medium (IMDM) medium (Thermo Scientific, #12200069) supplemented with 10 % FBS and P/S

Ligands of the asialoglycoprotein receptor for targeted gene delivery, part 1: synthesis of and binding studies with biotinylated cluster glycosides containing N-acetylgalactosamine

doi: 10.1016/0014-5793(96)00272-4

found that 6 azoospermia patients had a deletion of the long arm of the Y chromosome, and therefore, It is hypothesized that there is a gene controlling spermatogenesis in the nonfluorescent region of the long arm of the Y chromosome (Yq11.23), which is named azoospermia factor because this abnormality is present in many men with azoospermia (158) ( Figure 4B )

In Gerbera hybrida (Asteraceae), a TRV-based VIGS system was successfully created, allowing for quick functional tests on this transformation-resistant plant