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Total RNA extraction and RT-qPCR Total RNA was extracted from tissues and cultured cells using the RNAiso Plus reagent (Takara Bio, Tokyo, Japan) in accordance with the manufacturers protocol

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This manuscript provided the first evidence for the use of a receptor tyrosine kinase inhibitor, imatinib, as a disease-directed therapy for a specific variant of hypereosinophilic syndrome

doi: 10.5772/intechopen.91827 90
