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Ex vivo vascular reactivity studies Mice were anesthetized with isoflurane, and descending thoracic aortas were immediately isolated and dissected into 2 mm rings ( n = 8 per mouse) in ice-cold physiological salt solution (PSS

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The membranes were prehybridized at 60C for 2 hours, then hybridized at 60C for 10 hours, and washed with wash solution twice for 15 minutes

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